Multi-platform analysis of the tumor immune microenvironment associated with breast cancer subtypes.

ABSTRACT: Breast cancer is genetically and histologically heterogenous, and is influenced by a variety of factors, including the tumor microenvironment (TME). The PAM50 subtypes; Luminal A, Luminal B, Normal-like, Basal-like and Her2-enriched, are associated with different tumor phenotypes and overall survival. The quantity and quality of immune cell infiltration in breast tumors play a key role in cancer development and progression and are associated with survival and treatment response. We used multiplex immunohistochemistry, single-cell RNA sequencing, and two deconvolution algorithms to explore the immune landscape across PAM50 subtypes. Immunostaining of CD3⁺ T cells, tryptase⁺ mast cells, CD20⁺ B cells, CD68⁺ CD163⁺ macrophages, and CD66b<

SUBMITTER: Torland LA 

PROVIDER: S-EPMC12818823 | biostudies-literature | 2026 Dec

REPOSITORIES: biostudies-literature