KymoTip: high-throughput characterization of tip-growth dynamics in plant cells.

ABSTRACT: Live imaging data analysis often requires an objective, local, and accurate way of quantification of cell dynamics. In the research field of polarized tip-growth, the cell fluctuations and/or fluctuations in tip position and growth direction hamper automated analyses of huge amounts of imaging sequences. The fluctuated nature in data makes it unclear how cell shape and growth are linked to intracellular events that could be the actual driving force of cell growth. To overcome these difficulties, we developed a powerful and user-friendly tool called KymoTip with an available format. In this software, novel functions such as coordinate normalization, tip-bottom detection, and signal kymograph were implemented. We confirmed that not only plasma membrane-labeled fluorescent images, but also im

SUBMITTER: Kang Z 

PROVIDER: S-EPMC12818910 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature