Proteomic analysis of differential biological responses and fatty acid metabolism in slow-growing chickens fed dietary tuna oil directly or via glucose transporter-targeted nanoparticles.

ABSTRACT: This study investigated the effects of tuna oil (PC) and tuna oil encapsulated in lipid nanoparticles (TNP) on metabolic pathways in thigh muscle of slow-growing chickens using proteomic analysis. Compared with the negative control (NC) diet containing 6% rice bran oil, the PC and TNP diets replaced half of the rice bran oil with tuna oil, thereby increasing dietary n-3 PUFAs and altering the n-6/n-3 ratio. Iliotibialis muscle samples were collected from slow-growing Korat chickens (n = 6 for each PC and TNP; n = 5 for NC). Fatty acid profiles were analyzed by gas chromatography-mass spectrometry following lipid extraction and methylation. Proteomics analysis was performed using polyacrylamide gel-based protein separation and nanoLC-MS/MS to identify and quantify differentially expressed p

SUBMITTER: Homyok P 

PROVIDER: S-EPMC12819037 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature