High-throughput peptide-centric local stability assay extends protein-ligand identification to membrane proteins, tissues and bacteria.

ABSTRACT: Systematic mapping of protein-ligand interactions is essential for understanding biological processes and drug mechanisms. Peptide-centric local stability assay (PELSA) is a powerful tool for detecting these interactions and identifying potential binding sites. However, its original workflow is limited in throughput, sample compatibility and accessible protein targets. Here, we introduce a high-throughput adaptation-HT-PELSA-that increases sample processing efficiency by 100-fold while maintaining high sensitivity and reproducibility. HT-PELSA substantially extends the capabilities of the original method by enabling sensitive protein-ligand profiling in crude cell, tissue and bacterial lysates, allowing the identification of membrane protein targets in diverse biological systems. We demons

SUBMITTER: Li K 

PROVIDER: S-EPMC12819154 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature