Cangfu Daotan decoction treats PCOS-IR through the IL6/JAK2/STAT3/FOXO4 signaling pathway.

ABSTRACT:

Objective

This study aimed to investigate the protective effects and underlying mechanisms of Cangfu Daotan Decoction (CDD) in both vivo and in vitro models of polycystic ovary syndrome with insulin resistance (PCOS-IR).

Materials and methods

Active compounds in CDD were identified using UPLC-HRMS. Network pharmacology and molecular docking analyses were employed to predict key molecular targets. A nd a high-fat diet. In vitro, KGN cells were used to simulate granulosa cell dysfunction associated with PCOS-IR. The regulatory effects of CDD on the IL6/JAK2/STAT3/FOXO4 signaling pathway were further evaluated.

Results

Fifteen active compounds in CDD were preliminarily identified. Ninety-four potential target genes related to the treatment of PCOS-IR

SUBMITTER: Ju W 

PROVIDER: S-EPMC12819200 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature