Evaluation of the diagnostic value of metagenomic next-generation sequencing for zoonotic cestode <i>Spirometra mansoni</i> infection.

ABSTRACT: Metagenomic next-generation sequencing (mNGS) technology offers substantial advantages in parasite detection; however, we still know very little about its diagnostic value for Spirometra mansoni infection. In this study, mNGS technology was used to analyse faecal samples and blood samples from cats infected with S. mansoni, as well as tissue samples and blood samples from mice infected with the plerocercoid larvae of S. mansoni. Moreover, polymerase chain reaction (PCR) was employed to validate the mNGS results. The diagnostic value of mNGS for S. mansoni infection was systematically evaluated. The mNGS results revealed that the read counts of S. mansoni in the cat faeces (CF) samples were 301,497 (CF1), 1,330,549 (CF2), 1,181,162 (CF3), and 0 (CF0), with

SUBMITTER: Ru SS 

PROVIDER: S-EPMC12819346 | biostudies-literature | 2026 Mar

REPOSITORIES: biostudies-literature