Fast and efficient intracellular delivery of large size nanoparticles into mammalian cells by in situ electroporation.

ABSTRACT: Nanoparticles of various sizes and materials are widely used for targeted intracellular delivery in mammalian cell models. However, endocytic uptake often leads to endosomes embedding, reducing delivery efficiency, especially for larger particles. Electroporation offers a direct means of accessing the cytosol, bypassing this limitation. Here, we employ a microchip featuring integrated capacitive microelectrodes to perform spatially selective, rapid and efficient electroporation of 200 nm fluorescent nanoparticles into adherent CHO-K1 epithelial cells. Operating at low voltage amplitudes, the system minimizes cellular damage while maintaining high delivery efficiency. We confirm effective cytoplasmic delivery and preferential perinuclear localization of internalized nanoparticles, with mini

SUBMITTER: Maschietto M 

PROVIDER: S-EPMC12819381 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature