OxyR contributes to the oxidative stress capacity and virulence of hypervirulent <i>Klebsiella pneumoniae</i> ATCC 43816.

ABSTRACT:

Introduction

Hypervirulent Klebsiella pneumoniae (hvKP) is an emerging pathogen associated with severe invasive infections and high mortality, in which resistance to host-derived reactive oxygen species (ROS) is critical for immune evasion and persistence. However, the mechanisms underlying oxidative stress resistance in hvKP remain poorly understood, and the role of the global regulator OxyR in this species has not been fully elucidated.

Methods

In this study, VK055_RS16305 was first identified as an OxyR homologue in K. pneumoniae ATCC 43816 by sequence alignment. The oxyR deletion mutant was generated using a CRISPR/Cas9-based genome editing system, whereas the complemented strain was obtained using the pSTV28 plasmid carrying oxyR. We then com

SUBMITTER: Zhang R 

PROVIDER: S-EPMC12819678 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature