Opposing fates: a bipolar cellular model for FMDV replication shaped by ¹²C⁶⁺heavy-ion mutagenesis.

ABSTRACT:

Introduction

By pioneering the use of an 80 MeV/u ¹²C⁶⁺ heavy-ion beam for mutagenesis, we have engineered a stably polarized BHK-21 cell model for FMDV replication.

Methods

This approach yielded two distinct clones: a highly antiviral line (BHK-5) and a highly proviral line (BHK-7). Multi-omics analyses were employed to investigate the mechanisms driving these divergent phenotypes.

Results

The divergent phenotypes stem from a profound reprogramming of host transcriptional networks. The antiviral BHK-5 clone exhibits a pre-activated innate immune state, leveraging RIG-I/TLR signaling for a rapid interferon response and viral clearance via autophagy. In stark contrast, the proviral BHK-7 clone enhances glycolysis and activates the PI3K-Akt

SUBMITTER: Song X 

PROVIDER: S-EPMC12819685 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature