Whole-genome identification of <i>LdELF4s</i> and analysis of their expression response to diurnal temperature variations in <i>Lilium davidii</i> var. <i>willmottiae</i> (E. H. Wilson) raffill.

ABSTRACT:

Introduction

Lanzhou lily (Lilium davidii var. willmottiae), a medicinal and edible plant endemic to China, synthesizes bioactive compounds regulated by diurnal temperature variations (DIFs). ELF4 family genes, key regulators coordinating endogenous rhythms with external changes, are unexplored in this species.

Methods

We analyzed nuclear-cytoplasmic phylogeny across 11 species and identified ELF4 homologs. Phylogenetic, collinearity, cis-element, and expression pattern analyses were conducted for Lanzhou lily ELF4s (LdELF4s). qRT-PCR assessed LdELF4 expression under different DIFs (20/20°C, 25/10°C, 20/5°C day/night), and corresponding phenotypes were evaluated.

Results

Cytonuclear discordance was observed within Lilium

SUBMITTER: Ma X 

PROVIDER: S-EPMC12819689 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature