Red light-driven enhancement of calycosin biosynthesis in Astragalus membranaceus via functional characterization of AmI3'H.

ABSTRACT: Astragalus membranaceus is a well-known medicinal plant rich in bioactive compounds, particularly the isoflavonoid calycosin, which exhibits diverse pharmacological properties. However, there is limited research on the influence of light conditions on calycosin biosynthesis and the underlying molecular mechanisms. In this study, we exposed in vitro plantlets of A. membranaceus to different LED light sources (white, red, and blue) and found that red light treatment significantly enhanced both biomass accumulation and calycosin content. Through transcriptome analysis combined with real-time PCR we identified several key genes involved in calycosin biosynthesis, among which AmI3'H showed the most significant upregulation under red light. Structural analysis and molecular docking indicated tha

SUBMITTER: Seo JW 

PROVIDER: S-EPMC12820219 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature