Capacitation-Induced Zinc Ion Flux and Sperm Plasma Membrane Remodeling Predict Porcine In Vitro Fertilization Cleavage Success.

ABSTRACT: Semen evaluation in human and animal reproduction relies on sperm motility and morphology; however, these often fail to predict fertility. The domestic boar (Sus scrofa) serves as a biomedical model for male reproduction due to similarities in sperm size, capacitation dynamics, and acrosomal structure to humans in comparison to traditional rodent models. This study evaluated sperm capacitation biomarkers, particularly zinc signatures, to predict cleavage success after in vitro fertilization (IVF). Semen from 20 boars (3 replicates each) was analyzed at 0, 1 and 4 h post-in vitro capacitation (IVC) using image-based flow cytometry to assess 4 zinc signatures, plasma membrane integrity, and acrosomal remodeling. Capacitation kinetics were quantified between timepoints. Motility was measured

SUBMITTER: Rodriguez I 

PROVIDER: S-EPMC12820604 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature