Reactive fluorescent probe for covalent membrane-anchoring: enabling real-time imaging of protein aggregation dynamics in live cells.

ABSTRACT: Aberrant aggregation of membrane proteins is a pathological hallmark of various diseases, including neurodegenerative disorders and cancer. The visualization of membrane protein aggregation has emerged as an important approach for investigating protein structure and function and for studying disease mechanisms and therapeutic interventions. While significant progress has been made in modifying membrane proteins and studying related biological processes, membrane protein aggregation remains underexplored, largely due to the lack of simple and effective methods for directly labeling native proteins and tracking this process in real time. With this research, we present a fluorescent probe equipped with a membrane-anchoring unit and a covalent reactive moiety for visualizing membrane protein d

SUBMITTER: Wei H 

PROVIDER: S-EPMC12820711 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature