Cryo-EM reveals an ensemble of cytochrome P450 reductase conformations in solution.

ABSTRACT: The eukaryotic electron transport system, mediated by cytochrome P450 reductase (CPR), plays a crucial role in driving myriads of reactions involved in the biosynthesis of physiologically active compounds (such as sterols, steroids, vitamins, and natural products), as well as in the metabolism of drugs, toxins, and carcinogens. CPR is a diflavin-containing enzyme found ubiquitously on the cytosolic side of the endoplasmic reticulum. While several crystal structures of CPR are available, its conformational states in solution, along with the molecular details of action, remain debatable. Here, we determined the 3.3 Å cryo-EM structure of rat CPR, marking the first electron microscopy structure of this relatively small protein (77 kDa). In this structure, the full-length, fully active enzyme

SUBMITTER: Lepesheva GI 

PROVIDER: S-EPMC12820792 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature