Coordinated immune networks in leukemia bone marrow microenvironments distinguish response to cellular therapy.

ABSTRACT: Understanding how intratumoral immune populations coordinate antitumor responses after therapy can guide treatment prioritization. We systematically analyzed an established immunotherapy, donor lymphocyte infusion (DLI), by assessing 348,905 single-cell transcriptomes from 74 longitudinal bone marrow samples of 25 patients with relapsed leukemia; a subset was evaluated by both protein- and transcriptome-based spatial analysis. In acute myeloid leukemia (AML) DLI responders, we identified clonally expanded ZNF683⁺ CD8⁺ cytotoxic T lymphocytes with in vitro specificity for patient-matched AML. These cells originated primarily from the DLI product and appeared to coordinate antitumor immune responses through interaction with diverse immune cell types within the ma

SUBMITTER: Maurer K 

PROVIDER: S-EPMC12820906 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature