Impact of Arsenite on Transient and Persistent Histone H3 Modifications and Transcriptional Response.

ABSTRACT: Arsenite-contaminated groundwater poses a major health concern affecting millions of people. Chronic exposure to elevated levels of inorganic arsenic is implicated in carcinogenesis, with impaired DNA repair and dysregulated DNA and histone modifications as key factors. Using human A549 lung carcinoma cells, we investigated the persistence of acute arsenite-induced cellular stress at the epigenetic and transcriptional levels after 24 h of exposure to 1-25 μM NaAsO₂, reflecting low to high acute exposure scenarios, followed by a 48 h arsenite-free postincubation period. The primary objective was to analyze alterations in acetylation and methylation marks on both bulk histone H3 and specific DNA repair gene loci. We conducted immunochemical and proteomic analyses to assess alterat

SUBMITTER: Lumpp T 

PROVIDER: S-EPMC12820973 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature