Self-Assembled Hydrogel from Pyrene-Modified Peptide as 3D Matrices for Neuronal Cell.

ABSTRACT: Self-assembled hydrogels offer biomimetic platforms for three-dimensional (3D) cell culture; yet, achieving stability and functionality under physiological conditions remains a challenge. Here, we report a series of pyrene (Py)-modified peptides designed to form hydrogels with tunable physical and biological properties. Among them, Py-L₃K₃ uniquely formed stable, fluorescent hydrogels at both neutral and basic pH, in contrast to Py-K₆ and Py-A₃K₃, which gelled only at basic pH. Molecular dynamics simulations revealed pH-dependent clustering as a key mechanism driving hydrogel formation. Structural analysis showed that Py-L₃K₃ forms nanofibrillar networks with granular surface morphologies and β-sheet-rich conforma

SUBMITTER: Wahyuningtyas D 

PROVIDER: S-EPMC12820978 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature