Time-Resolved Forster Resonance Energy Transfer Nanoassay Based on CdTe Quantum Dots for Sensitive Detection of Prostate Cancer Antigen 3.

ABSTRACT: Prostate Cancer Antigen 3 (PCA3) is a long noncoding RNA highly expressed in prostate cancer cells, making it a promising biomarker for noninvasive prostate cancer diagnosis. Simple and rapid detection using nanoprobes can potentially overcome the limitations of traditional diagnostic techniques. Here, we designed, characterized, and applied a DNA-strand displacement assay based on Förster Resonance Energy Transfer (FRET) between terbium (Tb) ions and semiconductor quantum dots (QDs) as a proof-of-concept for sensitive and specific mix-and-measure quantification of a synthetic DNA analogue of PCA3. The assay utilized QDs synthesized through an aqueous one-pot method. The time-resolved (TR) FRET assays achieved a detection limit of 0.65 nmol L^-1 by using a SPARK benchtop f

SUBMITTER: Martins CSM 

PROVIDER: S-EPMC12821037 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature