Development of an impedimetric aptasensor for Shiga-like toxin 1 using a DNA aptamer and an interdigitated microelectrode.

ABSTRACT: The life-threatening diarrheal disease caused by Shiga toxin-producing Escherichia coli (STEC) necessitates rapid, sensitive, portable, and low-cost diagnostic tools to strengthen on-field food safety monitoring, improve disease prognosis, and guide timely therapeutic interventions. In this study, an impedimetric aptasensor was developed against Shiga like toxin-1 produced by STEC using a 71-mer ssDNA aptamer (sT3), enriched from an initial library (∼10¹⁴ sequences) through the Systematic Evolution of Ligands by Exponential Enrichment (SELEX) technique, and an interdigitated chain-type microelectrode fabricated by depositing Ti and Au layers on a glass substrate via electron-beam deposition, photolithography, and wet-chemical etching. The aptamer exhibited a strong

SUBMITTER: Mili M 

PROVIDER: S-EPMC12821148 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature