An optimized protocol for plant extracellular vesicles isolation from Ophiopogon japonicus root: a comparative evaluation based on miRNA cargo.

ABSTRACT:

Background

Plant extracellular vesicles (EVs), hold significant therapeutic potential due to their roles in intercellular communication and cross-kingdom regulation, primarily mediated by their microRNA (miRNA) cargo. However, isolating high-purity plant EVs from complex plant tissues, such as the tuberous roots of Ophiopogon japonicus, is challenging due to the dense cell wall matrix and high content of contaminants like polysaccharides. Existing isolation methods, including differential ultracentrifugation (DUC) and density gradient ultracentrifugation (DGUC), involve trade-offs between yield, purity, and vesicle integrity, necessitating the development of optimized protocols.

Results

We developed and systematically optimized an integrated protocol for isolating high-purit

SUBMITTER: Xiao Y 

PROVIDER: S-EPMC12821176 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature