Studies of the motifs that regulate subcellular localization reveal differences between the homologous neuronal proteins NeuroD1 and NeuroD2.

ABSTRACT:

Background

NeuroD1 and NeuroD2, members of the bHLH transcription factors family, are key regulators of nervous system development and function. While they share similar roles in neuronal regulation, their divergent expression patterns and specialized functions suggest the complexity of transcriptional control they perform. The activity of bHLH TFs depends on tightly regulated intracellular trafficking orchestrated by NLSs and/or NESs located within the protein's sequences. A detailed characterization of these molecular motifs is essential to understand the mechanisms regulating NeuroD1 and NeuroD2 functions.

Methods

We prepared cDNA vector that enabled expression of the full-length and truncated variants of NeuroD1 and NeuroD2 fused to YFP in COS-7 and N2a cells. Confocal m

SUBMITTER: Kolonko-Adamska M 

PROVIDER: S-EPMC12821249 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature