Analysis of high glucose injury using human induced pluripotent stem cell-derived kidney organoids.

ABSTRACT:

Background

Diabetic kidney disease (DKD) is one of the most pervasive complications of diabetes worldwide. However, the pathogenesis of DKD remains poorly understood, due to limitations of the models. The hPSC-derived kidney organoids may offer a new possibility to solve the problem.

Methods

We generated human pluripotent stem cells (hPSCs) derived kidney organoids to model DKD injury by glucose intervention for 24 and 72 h, respectively. RT-qPCR was used to assess gene expression, while immunofluorescence was performed to evaluate protein expression. PAS staining was applied to examine organoid morphology, and Sirius Red staining was used to assess fibrosis.

Results

Firstly, qPCR results showed that glucose and lipid metabolism-related genes such as LDH, HK2, SGLT2,

SUBMITTER: Qian Z 

PROVIDER: S-EPMC12821291 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature