Comparative analysis of 105 datasets across species and tissues reveals differential transcriptomic responses to cannabinoids THC and CBD.

ABSTRACT:

Background

Cannabis use is on the rise yet the systematic molecular impact of key cannabinoid components on various tissues in diverse organisms remains incompletely understood. We aim to systematically elucidate the molecular pathways and networks affected by delta-9-tetrahydrocannabinol (THC) and cannabidiol (CBD) across species and tissue types.

Methods

We curated 105 THC- and CBD-related RNA sequencing (RNAseq) and microarray datasets from Gene Expression Omnibus (NCBI GEO) with a focus on mammalian species (human, non-human primate rhesus macaque, mouse, rat). Differentially expressed genes (DEGs) were identified using limma for microarrays and DESeq2 for RNAseq data, followed by a meta analysis to identify meta-DEGs. DEGs were analyzed for pathway enrichment using Enri

SUBMITTER: Liu R 

PROVIDER: S-EPMC12821297 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature