LAMC2 promotes gallbladder carcinoma metastasis through the TGF-β pathway.

ABSTRACT:

Background

Gallbladder cancer (GBC) is an aggressive malignant tumor that seriously threatens the survival of GBC patients. Laminin-γ2 (LAMC2), an important component of laminins, has been reported to facilitate cancer development and chemoresistance in several cancers. However, the biological effect of LAMC2 on GBC is still unclear.

Methods

LAMC2 expression was assessed in GBC specimens through proteomics and RNA-seq data. To evaluate the biological effects of LAMC2, we performed colony formation, sphere formation, wound healing, and transwell and invasion assays, along with an in vivo metastasis model. Additionally, we also explored the role of LAMC2 in GBC organoid. Finally, Western blot and RT-qPCR were employed to identify the molecular mechanism underlying LAMC2-mediat

SUBMITTER: Xu M 

PROVIDER: S-EPMC12821298 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature