Protective role of fatty acid oxidation against epithelial barrier dysfunction in allergic asthma.

ABSTRACT:

Background

Fatty acid oxidation (FAO) is implicated in lung diseases, but its role in bronchial asthma is not fully understood. We investigated its effect on airway epithelial barrier integrity.

Methods

Using a house dust mite (HDM)-induced murine asthma model and HDM, IL-4, IL-13, or TNF-α stimulated human primary bronchial epithelial cells (BECs) and bronchial epithelial (Beas-2b) cells, we modulated FAO with L-carnitine (agonist) and Etomoxir (inhibitor). BECs and Beas-2b cells were infected with lentivirus-mediated CPT1A shRNA prior to stimulation. Barrier function, mitochondrial oxidative stress, inflammation, and metabolism were assessed.

Results

FAO level in lungs negatively correlated with increased inflammation and tissue injury in HDM-induced asthmati

SUBMITTER: Wang M 

PROVIDER: S-EPMC12821354 | biostudies-literature | 2026 Dec

REPOSITORIES: biostudies-literature