Proteomics and Bioinformatics Profiles of Human Mesothelial Cell Line MeT-5A.

ABSTRACT: Background: Despite existing proteomics studies of other cell types, a comprehensive proteome of mesothelial cells has not been characterized. This study establishes a crucial baseline proteome for mesothelial cells to better understand their fundamental bioprocesses in healthy and injured states. Methods: Using mass spectrometry-based shotgun proteomics, we characterized the cellular fraction (CF) and conditioned medium (CM) proteomes of mesothelial cell line MeT-5A. The datasets were analyzed for Gene Ontology (GO) terms and canonical pathway enrichments to identify biological themes. Results: Our analysis identified 5087 protein groups, including 1532 shared proteins, 3122 unique to the CF and 433 exclusive to the CM. GO annotation revealed distinct functional enric

SUBMITTER: Watkin RL 

PROVIDER: S-EPMC12821629 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature