Callus and endosperm green fluorescence reporter-assisted selection system in maize CRISPR/Cas9 gene editing.

ABSTRACT:

Background

Genome editing using CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9) has emerged as a promising approach for functional gene analysis and genetic improvement. Since stable transformation remains the primary method for implementing this system, the ultimate goal in crop breeding programs would require the selection of transgene-free plants with the CRISPR/Cas expression cassette removed.

Results

In this study, we developed an endosperm-specific fluorescence reporter-assisted selection system for CRISPR/Cas9 gene editing (pAZS22-eGFP/CRISPR/Cas9) in maize (Zea mays L.), utilizing enhanced green fluorescent protein (eGFP) expressed specifically in the endosperm to facilitate the easy identification of transgenic and

SUBMITTER: Ge L 

PROVIDER: S-EPMC12821853 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature