Co-delivery of camptothecin and SH3RF3-AS1 SiRNA for glioma synergistic therapy.

ABSTRACT:

Background

Current treatment strategies for high-grade gliomas have a limited overall patient benefit. Here, Methylated RNA immunoprecipitation sequencing (MeRIP-seq) of glioma was conducted to screen the lncRNA regulated by m6A modification in glioma, and provide an innovative chemotherapeutic strategy to improved the therapeutic efficacy.

Methods

To elucidate the impact and the molecular mechanism of ALKBH5/SH3RF3-AS1/SH3RF3 regulation on glioma malignancy, proliferation phenotypic assays, MeRIP-qPCR, RIP-qPCR and western blotting were used. Expressions and clinical significances of ALKBH5, SH3RF3-AS1, and SH3RF3 were assessed in tissue chip. Furthermore, we designed a nanodrug (Nano^PCPT/siRNA@U251) for the targeted co-delivery of CPT (targeting type Ⅰ topoisome

SUBMITTER: Wang S 

PROVIDER: S-EPMC12822114 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature