MiR-199b-5p inhibition improves inflammation and oxidative stress damage in OA-induced non-alcoholic fatty liver disease through targeting SIRT1.

ABSTRACT:

Objectives

Non-alcoholic fatty liver disease (NAFLD) is a chronic liver disease. This research investigates the role of miR-199b-5p in NAFLD development.

Method

This study enrolled 110 non-NAFLD patients and 90 NAFLD patients and collected their clinical data and serum samples. The level of miR-199b-5p was measured via qRT-PCR. Through experiments such as ELISA, biochemical assays, and CCK-8, the effects of miR-199b-5p on OA-induced inflammation, lipid metabolism, viability, and oxidative stress in HepG2 cells were analyzed. Meanwhile, the targeting relationship between miR-199b-5p and SIRT1 was verified through dual-luciferase assay and Western blot analysis.

Results

miR-199b-5p was highly expressed in the NAFLD group and was significantly correlated with BMI, WC, TG

SUBMITTER: Liang H 

PROVIDER: S-EPMC12822143 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature