Ex Vivo Spatiotemporal Characterization of Spermatogenesis in Mouse Testicular Organoids.

ABSTRACT: Testicular organoids (TOs) offer an opportunity to preserve fertility, but current TO protocols are limited by suboptimal maintenance of the organoid structure, meiotic defects, and incomplete in vitro spermatogenesis (IVS). Here, a strategy is developed for self-reconstitution of single-cell suspensions of neonatal mouse testes into TOs containing the major testicular cell types and generation of tubular-like structures and haploid spermatids. Morphological and lineage-specific marker analyses revealed that these TOs met the criteria for organoids, including germ cell differentiation and recapitulation of key events in meiosis (e.g., chromosome recombination and synapsis). Notably, the spatiotemporal characteristics of spermatogenesis in the TOs are comparable to those in testes, and thei

SUBMITTER: Sun J 

PROVIDER: S-EPMC12822388 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature