Brillouin Microscopy of Breast tumor Spheroids On-a-Chip: Mechanical and Transcriptional Responses to Microfluidic-Induced Rapid Deformations.

ABSTRACT: Pioneering a novel integrated photonic platform, Brillouin-Raman Microspectroscopy on-a-Chip is presented, which enables real-time, high resolution analysis of mechano-chemical dynamics in complex 3D biological systems under controlled deformation. This contact-free, all-optical method seamlessly combines Brillouin and Raman microscopy within microfluidic devices, providing a unique correlative approach to overcome current limitations in probing cellular mechanics and biochemical responses in live multicellular models. This innovative platform, when applied to breast tumor spheroids, revealed that rapid, cyclical mechanical deformations, mimicking in vivo stresses, profoundly impacts tumor physiology. These findings demonstrate that controlled deformations trigger rapid nuclear shape chang

SUBMITTER: Makkieh M 

PROVIDER: S-EPMC12822390 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature