A Dynamic DNA Nano-Antioxidant Targeting Galectin-3 Attenuates Liver Fibrosis via Reducing Macrophage Oxidative Stress.

ABSTRACT: Liver cirrhosis represents a major global health challenge with significant socioeconomic implications. Oxidative stress-mediated injury plays a pivotal role in driving fibrotic progression, with hepatic macrophages serving as a dominant source of reactive oxygen species (ROS). Consequently, targeted suppression of macrophage-derived ROS presents a promising therapeutic strategy. Single-cell RNA sequencing analysis identified Galectin-3 as positively correlated with macrophage oxidative stress and ROS production. Here, a dynamic DNA nano-antioxidant (DDN) is developed for hepatic macrophage-specific small interfering RNA (siRNA) delivery targeting Galectin-3. DDN administration achieved a 3.92-fold reduction in Galectin-3 expression, with transcriptomic profiling revealing restoration of N

SUBMITTER: Peng M 

PROVIDER: S-EPMC12822432 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature