SIRT2 Regulates Ex Vivo PBMC Adhesion in Septic Shock Patients.

ABSTRACT:

Background

Septic shock (SS) is deadly. Sepsis-immune response transitions from an endotoxin-sensitive, hyper-inflammatory phase to an endotoxin-tolerant hypo-inflammatory phase. In mice, we implicated sirtuin 2 (SIRT2) for prolonged hypo-inflammation using leukocyte adhesion, the earliest in vivo inflammatory response to cytokine, chemokine, and metabolite stimuli. The role of SIRT2 in human sepsis remains unknown. We hypothesized that: 1. Peripheral blood mononuclear cells (PBMCs) adhesion response can be used as a physiological biomarker of hypo-inflammation, and 2. SIRT2 regulates the functions of PBMCs and macrophages during the hypo-inflammatory phase of human sepsis.

Methods

We stimulated control and SS-whole blood and PBMCs ± lipopolysaccharide (LPS) and investigated

SUBMITTER: Shrestha D 

PROVIDER: S-EPMC12823044 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature