Mass Determination of Filled and Empty AAV5 Particles Enabled by Nanoelectrospray Ionization and Proton Transfer Charge Reduction.

ABSTRACT: The mass determination of bio-ions that exceed a megadalton poses many challenges. While it is possible to generate gaseous ions from large biocomplexes, including intact viruses, via nanoelectrospray ionization (nESI), generating mass information using conventional ensemble measurements (i.e., from conventional mass spectra) requires the resolution of charge states. As biocomplexes increase in size, overlap of adjacent charge states becomes increasingly problematic. Single ion measurements that enable the simultaneous determination of mass/charge and charge can overcome the charge state overlap problem. However, ensemble measurements are, in principle, much faster. We demonstrate here the mass determination of empty and filled adeno-associated virus particles, serotype 5 (AAV5), both sepa

SUBMITTER: Pizzala NJ 

PROVIDER: S-EPMC12823214 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature