Development and validation of a sensitive fluorescence RT-qPCR assay with TaqMan-MGB probe for detection of H6 subtype avian influenza A virus.

ABSTRACT: Avian influenza, caused by the avian influenza A virus (IAV), threatens poultry and public health. H6 subtype avian IAV is a low-pathogenic virus with hosts ranging from poultry and wild birds to mammals. H6 persists latently in poultry, which enables silent transmission and cross-species risk. The few fluorescence quantification assays that exist for H6 are mostly multiplexed. We developed a rapid, sensitive, efficient, monoplex fluorescence reverse-transcription qPCR (RT-qPCR) assay for H6 IAV. Specific primers and a TaqMan-MGB probe were designed based on the conserved hemagglutinin (HA) gene region of H6 IAVs from the GISAID database. The reaction components and conditions were optimized, and the assay was evaluated for specificity, sensitivity, and reproducibility. The optimized assay

SUBMITTER: Li X 

PROVIDER: S-EPMC12823365 | biostudies-literature | 2026 Mar

REPOSITORIES: biostudies-literature