A lipid nanoparticle encapsulated CPA-CTD mRNA vaccine provides protection against <i>Clostridium perfringens</i>-driven diseases.

ABSTRACT:

Introduction

Clostridium perfringens (C. perfringens), a ubiquitous Gram-positive bacterium in the environment and mammalian gut flora, is a leading cause of enterotoxemia in animals, necrotizing enteritis in humans and animals, and gas gangrene in both, attributed to its diverse exotoxin profile. Alpha-toxin, a pivotal virulence factor produced by all C. perfringens serotypes, plays a central role in the pathogenicity of these diseases.

Methods

Here, we engineered a lipid nanoparticle encapsulated CPA-CTD mRNA vaccine targeting the conserved C-terminal domain of C. perfringens alpha-toxin and rigorously assessed its immunogenicity and protective efficacy in mouse and bovine models.

Results

The CPA-CTD mRNA vaccine induced strong humoral an

SUBMITTER: Jia Q 

PROVIDER: S-EPMC12823533 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature