Modulation of IRAK4 as a therapeutic strategy against monosodium urate- and xanthine-induced inflammation in macrophages and HepG2 cells.

ABSTRACT:

Background

Interleukin-1 receptor-associated kinase 4 (IRAK4) is a pivotal mediator of Toll-like receptor (TLR) and interleukin-1 receptor (IL-1R) signaling, critically involved in innate immune activation and pro-inflammatory cytokine production. Dysregulated IRAK4 activity contributes to chronic inflammation in both immune and non-immune cells. In this study, we evaluated the immunomodulatory potential of a selective IRAK4 inhibitor on monosodium urate (MSU) crystal-stimulated macrophages and xanthine-challenged HepG2 cells to assess its therapeutic potential.

Methods

Human peripheral blood mononuclear cells (PBMCs) were pretreated with 1 µM IRAK4 inhibitor (IRAK4i) overnight, followed by stimulation with 100 µg/mL of MSU for either 30 min or 24 h. Conditioned medium was c

SUBMITTER: Umar S 

PROVIDER: S-EPMC12823803 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature