Investigation on the fluorescence detection mechanism of NIR fluorescent probes based on intramolecular spiro cyclization.

ABSTRACT:

Introduction

This study focuses on the detection mechanisms of recently developed NIR fluorescent probes that depend on ring formation and opening processes. A novel class of polymethine dyes (NIRII-RTs) serves as the core fluorescent moiety of these probes, which exhibit bright, stable, and anti-solvent quenching NIR-II emission, accompanied by large Stokes shifts.

Methods

Quantum chemical calculation methods were employed to systematically analyze the light absorption and emission processes of three target-specific probes: NIR-pH (targeting H⁺), NIR-ATP (targeting ATP), and NIR-Hg (targeting Hg²⁺).

Results

The results demonstrated that the probes exhibit weak fluorescence in the closed spiro cyclization state. This weak emission is attributed t

SUBMITTER: Zhang ZW 

PROVIDER: S-EPMC12823956 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature