Long-term evaluation of human iPSC-derived cartilage for repairing chondral defects.

ABSTRACT: Induced pluripotent stem cells (iPSCs) have demonstrated superior capacity to regenerate hyaline cartilage compared to mesenchymal stromal cells (MSCs). However, most previous animal studies have only conducted short-term assessments. We performed a long-term (8 weeks) in vitro chondrogenesis of human iPSC-derived multipotent progenitor cells (iMPCs) and human MSCs. The expression levels of hypertrophy-related genes were significantly lower in the iMPC group compared to the MSC group, such as collagen type X being 5-fold lower on day 56. In the animal study, implants from the iMPC group maintained more matrix than the MSC group at both short and long-term time points (12 and 48 weeks). Importantly, at 48 weeks, the native cartilage surrounding the defect areas in some rats from the MSC gro

SUBMITTER: Lu Y 

PROVIDER: S-EPMC12824314 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature