Antigen binding triggers long-range conformational changes in monoclonal antibodies.

ABSTRACT:

Introduction

Antigen binding in monoclonal antibodies (mAbs) is traditionally associated with target recognition, but emerging evidence suggests it may also modulate antibody conformation and effector functions. Understanding how antigen engagement impacts the structural organization of therapeutic antibodies and their interaction with immune receptors is essential for optimizing antibody-based therapies. In this study, we investigated, via computational approaches, how antigen engagement alters the structural organization of therapeutic IgG1s and influences their ability to interact with FcγRIIIa.

Methods

Accelerated molecular dynamics simulations were performed to investigate the structural and dynamic consequences of antigen binding in two therapeutic mAbs, adalimu

SUBMITTER: Bianchi D 

PROVIDER: S-EPMC12824876 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature