MiR-539-5p Regulates Irritable Bowel Syndrome Pathological Processes by Targeting KDM6A.

ABSTRACT: Background/Aims: The chronicity and recurrence of irritable bowel syndrome (IBS) pose significant burdens on patients' lives, making it urgent to understand its underlying mechanisms. This study intends to investigate the function and regulatory mechanisms of miR-539-5p in IBS and lay the foundation for the creation of more effective therapeutic strategies. Materials and Methods: Rat model of IBS with diarrhea (IBS-D) was established and evaluated by the abdominal withdrawal reflex. The IBS cellular model was established in vitro using lipopolysaccharide (LPS), and real-time quantitative polymerase chain reaction was used to assess the changes in the miR-539-5p expression. Cell counting kit-8 assays, flow cytometry, and enzyme-linked immunosorbent assay were used to evaluate the effects of

SUBMITTER: Li Y 

PROVIDER: S-EPMC12824890 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature