Isolation of small extracellular vesicles by interacting with inorganic surface.

ABSTRACT: Extracellular vesicles (EVs) have great potential as diagnostic and therapeutic tools because they are important mediators of intercellular communication. Although several EV isolation methods have been developed, efficient and selective isolation remains challenging owing to the presence of coexisting proteins and other EV subtypes. Herein, we report an EV purification method using inorganic materials composed of calcium phosphate and calcium carbonate combining with magnetic particles, wherein EVs are captured to phosphate and carbonate groups via surface calcium residues through metal coordinate bonds with their negatively charged phospholipids of EVs.

Methods

Cultured supernatants from three cell lines (HEK293T and cancer cells, MCF7 and PC3) were subjected to sequential centrif

SUBMITTER: Obuchi S 

PROVIDER: S-EPMC12824912 | biostudies-literature | 2026 Mar

REPOSITORIES: biostudies-literature