Efficient 2G Ethanol Production via Optimized Dilute Acid Pretreatment, High-Solids Enzymatic Hydrolysis, and High-Temperature Fermentation.

ABSTRACT: The growing demand for 2G ethanol emphasizes the need to improve its economic sustainability through different approaches, including optimized pretreatments, high-solids enzymatic hydrolysis, and high-temperature fermentation. This study evaluated the dilute acid pretreatment of deacetylated rice straw using the response surface methodology. Under optimal conditions (85 mg of H₂SO₄/g biomass, for 10 min at a constant temperature of 150 °C), the process was scaled up to an 80 L reactor, obtaining a cellulose-rich (58.2% w/w) pretreated solid. Then, high-solids enzymatic hydrolysis at 24% w/v solids loading was performed in a vertical ball mill (VBM) reactor in fed-batch mode, resulting in a hydrolysate of 129 g/L of fermentable sugars, which corresponded to a cellulose

SUBMITTER: Ferreira IS 

PROVIDER: S-EPMC12824952 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature