Workflow for the Isolation and Characterisation of Human Milk Extracellular Vesicles (HMEVs) and Their Inflammatory Biomarker Profile.

ABSTRACT: Human milk extracellular vesicles (HMEVs), secreted by mammary epithelial cells, are enriched in bioactive molecules that support intestinal epithelial integrity. Among these, oxylipins, that is, lipid mediators derived from polyunsaturated fatty acids, are gaining interest for their immunomodulatory and neuroprotective functions in breastfed infants. However, current workflows for oxylipin profiling in HMEVs often lack sensitivity or breadth, limiting mechanistic insights. This study presents an optimised workflow for comprehensive oxylipin profiling in HMEVs. HMEVs were isolated via size-exclusion chromatography and ultracentrifugation, followed by characterisation using attenuated total reflectance-Fourier transform infrared spectroscopy, Western blotting, Exoview immunocapture, tunable

SUBMITTER: Moreno-Casillas JL 

PROVIDER: S-EPMC12825023 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature