Jub-induced phosphorylation of Warts inhibits its activity and recruitment into biomolecular condensates.

ABSTRACT: Biomolecular condensates play important roles in cellular physiology. Within the Hippo signaling pathway, a key regulator of growth and cell fate, condensates formed by upstream scaffolds recruit and promote the activation of the central kinase of the pathway, Warts (Wts)/LATS. Here, we report that the Wts inhibitor Jub also makes condensates that recruit Wts, raising the question of how partitioning of Wts to activating versus inhibitory condensates might be regulated. We address this by showing that Jub also promotes phosphorylation of the N-terminal intrinsically disordered region of Wts, and that N-terminal phosphorylation sites influence both Wts recruitment into condensates and Wts activity. We identify Minibrain and Homeodomain-interacting protein kinase as kinases that promote the

SUBMITTER: Kirichenko E 

PROVIDER: S-EPMC12825121 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature