3D Quantification of Viral Transduction Efficiency in Living Human Retinal Organoids.

ABSTRACT: The development of therapeutics builds on testing their efficiency in vitro. To optimize gene therapies, for example, fluorescent reporters expressed by treated cells are typically utilized as readouts. Traditionally, their global fluorescence signal has been used as an estimate of transduction efficiency. However, analysis in individual cells within a living 3D tissue remains a challenge. Readout on a single-cell level can be realized via fluorescence-based flow cytometry at the cost of tissue dissociation and loss of spatial information. Complementary, spatial information is accessible via immunofluorescence of fixed samples. Both approaches impede time-dependent studies on the delivery of the vector to the cells. Here, quantitative 3D characterization of viral transduction efficiencies

SUBMITTER: Rogler TS 

PROVIDER: S-EPMC12825330 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature