Deep Proteome Profiling of Rat Dorsal Striatal Synaptoneurosomes Following Methamphetamine Exposure.

ABSTRACT: Understanding how drug exposure alters synaptic proteins is essential for uncovering the molecular mechanisms underlying addiction and identifying new therapeutic targets. Synaptoneurosomes (SNs), subcellular fractions enriched in pre- and postsynaptic components, provide a powerful resource for studying synaptic adaptations in the brain. Here, we applied a deep, discovery-based proteomic strategy to profile SNs isolated from the dorsal striatum (DS) of rats 1 h after acute methamphetamine (Meth) administration. To maximize proteome coverage, we combined tandem mass tag (TMT) labeling, offline high-pH fractionation, and low-pH nanoflow liquid chromatography coupled to high-resolution (orbitrap) mass spectrometry (HRMS). This approach enabled identification of ∼6100 cytosolic and membrane p

SUBMITTER: Rodriguez LG 

PROVIDER: S-EPMC12825378 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature