Discovery of Seven ROS-Sensitive Immune Checkpoints and 46 Ligands Mediating Immune Suppression Through T cell-APC Networks.

ABSTRACT: Rationale: The functional landscape of immune checkpoints (ICs) operating on CD4⁺FoxP3⁺ regulatory T cells (Tregs) remain incompletely defined. Although canonical IC pathways are well characterized, the full spectrum of IC molecules governing Treg-mediated immune regulation across physiological and pathological contexts has not been fully explored. Methods: We performed a comprehensive, multi-dataset transcriptomic screening of Treg membrane proteins to identify candidate immune checkpoints. This approach yielded 151 putative novel ICs, including 45 Treg-specific molecules and 106 FoxP3⁺-upregulated candidates. Cross-referencing these candidates with ten well-established IC-deficient models refined the list to 85 high-confidence ICs. A subsequent high-stringency, integrating

SUBMITTER: Han B 

PROVIDER: S-EPMC12825432 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature