Avidin related protein 2 shows unique structural and functional features among the avidin protein family.

ABSTRACT:

Background

The chicken avidin gene family consists of avidin and several avidin related genes (AVRs). Of these gene products, avidin is the best characterized and is known for its extremely high affinity for D-biotin, a property that is utilized in numerous modern life science applications. Recently, the AVR genes have been expressed as recombinant proteins, which have shown different biotin-binding properties as compared to avidin.

Results

In the present study, we have employed multiple biochemical methods to better understand the structure-function relationship of AVR proteins focusing on AVR2. Firstly, we have solved the high-resolution crystal structure of AVR2 in complex with a bound ligand, D-biotin. The AVR2 structure reveals an overall fold similar to the previously

SUBMITTER: Hytonen VP 

PROVIDER: S-EPMC1282572 | biostudies-literature | 2005 Oct

REPOSITORIES: biostudies-literature