Raman spectroscopy reveals growth phase-dependent molecular differences in bacterial membrane vesicles.

ABSTRACT: Bacterial membrane vesicles (BMVs) have attracted significant attention as highly efficient transport vehicles for molecules crossing biological barriers and as key mediators in infection processes. Based on this increasing interest, the need for standardized isolation protocols and comprehensive analytical approaches becomes apparent. Here, we evaluated BMVs from the human pathogen Pseudomonas aeruginosa, isolated at six distinct growth phases, using physicochemical assays, functional characterization, and Raman spectroscopy. Conventional analyses revealed growth phase-dependent differences in protein content, surface charge, and immunogenicity. Raman spectroscopy provided detailed molecular fingerprints, identifying shifts in protein-to-lipid ratios, increased lipid saturation, an

SUBMITTER: Christe L 

PROVIDER: S-EPMC12826046 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature